Title: Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans Document date: 1991_12_2
ID: qrg1rtzi_12
Snippet: RNA was prepared from adult male rat tissues as described (2) . Indicated quantities of poly(A+) RNA were resolved on a 1% formaldehyde/agarose gel (26) and transferred by capillary blotting to a Zetaprobe membrane (Bio-Rad Laboratories) . Filters were prehybridized, hybridized, and washed as described (6) using the rat PCRl cDNA amplification product (26) as the radiolabeled probe ......
Document: RNA was prepared from adult male rat tissues as described (2) . Indicated quantities of poly(A+) RNA were resolved on a 1% formaldehyde/agarose gel (26) and transferred by capillary blotting to a Zetaprobe membrane (Bio-Rad Laboratories) . Filters were prehybridized, hybridized, and washed as described (6) using the rat PCRl cDNA amplification product (26) as the radiolabeled probe .
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