Title: In vitro analysis of the oligodendrocyte lineage in mice during demyelination and remyelination Document date: 1990_9_1
ID: vr5hnzp8_13
Snippet: The growth factors tested were bovine bFGF (10 ng/mi; R & D Systems, Minneapolis, MN), human PDGF (10 ng/mi; A and B chain heterodimer from R & D Systems), and human insulin-like growth factor I (IGF-I; 100 ng/mi; Amgen Biologicals, Thousand Oaks, CA). Each growth factor was added individually to a culture dish at 1 div with 0.5% FBS in defined medium (see above). Cultures were subsequently given a 20-h terminal pulse of [3I-I]thymidine and fixed.....
Document: The growth factors tested were bovine bFGF (10 ng/mi; R & D Systems, Minneapolis, MN), human PDGF (10 ng/mi; A and B chain heterodimer from R & D Systems), and human insulin-like growth factor I (IGF-I; 100 ng/mi; Amgen Biologicals, Thousand Oaks, CA). Each growth factor was added individually to a culture dish at 1 div with 0.5% FBS in defined medium (see above). Cultures were subsequently given a 20-h terminal pulse of [3I-I]thymidine and fixed at 3 die (see Fig. 1
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