Author: Girardi, Erika; Chane-Woon-Ming, Béatrice; Messmer, Mélanie; Kaukinen, Pasi; Pfeffer, Sébastien
Title: Identification of RNase L-Dependent, 3'-End-Modified, Viral Small RNAs in Sindbis Virus-Infected Mammalian Cells Document date: 2013_11_19
ID: v6uc0ijw_22
Snippet: In order to gain more insight into the nature of this modification, we treated our samples with calf intestinal phosphatase (CIP) (Fig. 3C, lanes 2, 4, 6, 8, 10, 12, 14 , and 16) prior to â¤-elimination (Fig. 3C, lanes 4, 8, 12, and 16) . The double treatment strongly affected the mobility of a control oligoribonucleotide with a 3= P (Fig. 3C, lane 4) , while the mobility of the 3= OH oligonucleotide was affected independently of the CIP treatme.....
Document: In order to gain more insight into the nature of this modification, we treated our samples with calf intestinal phosphatase (CIP) (Fig. 3C, lanes 2, 4, 6, 8, 10, 12, 14 , and 16) prior to â¤-elimination (Fig. 3C, lanes 4, 8, 12, and 16) . The double treatment strongly affected the mobility of a control oligoribonucleotide with a 3= P (Fig. 3C, lane 4) , while the mobility of the 3= OH oligonucleotide was affected independently of the CIP treatment (Fig. 3C, lanes 7 and 8) . Of note, the combined reactions changed neither the mobility of a control oligoribonucleotide bearing a CIP-insensitive modification (methyl group in position 2= of the last ribose) (Fig. 3C, lane 12) nor the mobility of SvsRNA-1 (Fig. 3C, lane 16) .
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