Author: Girardi, Erika; Chane-Woon-Ming, Béatrice; Messmer, Mélanie; Kaukinen, Pasi; Pfeffer, Sébastien
Title: Identification of RNase L-Dependent, 3'-End-Modified, Viral Small RNAs in Sindbis Virus-Infected Mammalian Cells Document date: 2013_11_19
ID: v6uc0ijw_27
Snippet: To further strengthen this observation and to identify the precise position of the nucleotide modification, we performed primer extension assays on RNA isolated from either SINV-infected HEK293 or Vero cells (Fig. 4C ). 2=-O-methylation can cause reverse transcriptase to pause one nucleotide before and/or at the O-methylated nucleotide. Indeed, we identified two bands (RT stops) that correspond to nucleotides G 8334 and A 8333 , the latter corres.....
Document: To further strengthen this observation and to identify the precise position of the nucleotide modification, we performed primer extension assays on RNA isolated from either SINV-infected HEK293 or Vero cells (Fig. 4C ). 2=-O-methylation can cause reverse transcriptase to pause one nucleotide before and/or at the O-methylated nucleotide. Indeed, we identified two bands (RT stops) that correspond to nucleotides G 8334 and A 8333 , the latter corresponding precisely to the 3= end of the SvsRNA-1 sequence (Fig. 4C) .
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