Author: Dejnirattisai, Wanwisa; Webb, Andrew I.; Chan, Vera; Jumnainsong, Amonrat; Davidson, Andrew; Mongkolsapaya, Juthathip; Screaton, Gavin
Title: Lectin Switching During Dengue Virus Infection Document date: 2011_6_15
ID: qos9vu3r_7
Snippet: The viral titers were determined by a focus-forming assay. Briefly, virus was serially diluted and incubated with Vero cells for 2 hours at 37°C. The monolayers were then overlaid with 1.5% carboxymethylcellulose and incubated at 37°C for 2 days. Virus foci were stained with anti-E antibody followed by peroxidase-conjugated anti-mouse immunoglobulin (Ig) and visualized by the addition of 3,3#-Diaminobenzidine tetrahydrochloride substrate......
Document: The viral titers were determined by a focus-forming assay. Briefly, virus was serially diluted and incubated with Vero cells for 2 hours at 37°C. The monolayers were then overlaid with 1.5% carboxymethylcellulose and incubated at 37°C for 2 days. Virus foci were stained with anti-E antibody followed by peroxidase-conjugated anti-mouse immunoglobulin (Ig) and visualized by the addition of 3,3#-Diaminobenzidine tetrahydrochloride substrate.
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