Author: FAN, Wen-Sheng; LI, He-Ming; HE, Yi-Ning; TANG, Ning; ZHANG, Li-Hua; WANG, Hai-Yong; ZHONG, Lian; CHEN, Jian-Cai; WEI, Tian-Chao; HUANG, Teng; MO, Mei-Lan; WEI, Ping
Title: Immune protection conferred by three commonly used commercial live attenuated vaccines against the prevalent local strains of avian infectious bronchitis virus in southern China Document date: 2018_7_18
ID: ty3cxcdt_13
Snippet: Peripheral blood mononuclear cells were isolated from another portion of the blood samples collected above and then used to analyze the percentages of the CD4 + and CD8 + T lymphocytes by flow cytometry. 1 × 10 6 cells were stained with Mouse Anti-Chicken CD4-PE and CD8a-FITC antibodies (Wuhan AmyJet Scientific Inc., Wuhan, China). All staining reactions were conducted following the manufacturers' protocols. Then the fluorescence positive cells .....
Document: Peripheral blood mononuclear cells were isolated from another portion of the blood samples collected above and then used to analyze the percentages of the CD4 + and CD8 + T lymphocytes by flow cytometry. 1 × 10 6 cells were stained with Mouse Anti-Chicken CD4-PE and CD8a-FITC antibodies (Wuhan AmyJet Scientific Inc., Wuhan, China). All staining reactions were conducted following the manufacturers' protocols. Then the fluorescence positive cells were analyzed by a BD Accuri TM C6 Flow Cytometer (Becton, Dickinson and Co., NJ, U.S.A.). doi: 10.1292/jvms.18-0249
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