Author: Crameri, Gary; Durr, Peter A.; Barr, Jennifer; Yu, Meng; Graham, Kerryne; Williams, Owen J.; Kayali, Ghazi; Smith, David; Peiris, Malik; Mackenzie, John S.; Wang, Lin-Fa
Title: Absence of MERS-CoV antibodies in feral camels in Australia: Implications for the pathogen's origin and spread Document date: 2015_11_2
ID: yxtepbta_19
Snippet: For the Luminex assay, there were only two samples giving a reading above 1000 MFI, the set cut-off for background binding. As shown in Table 2 , serum 20 has MFU readings of 1256 and 509 for SARS-CoV and MERS-CoV N proteins, respectively, while serum 54 has 205 and 1377, respectively. However, in comparison to the MFU readings of positive control sera (ranging from 8127 to 29,319), these low range "potentially positive" readings were considered .....
Document: For the Luminex assay, there were only two samples giving a reading above 1000 MFI, the set cut-off for background binding. As shown in Table 2 , serum 20 has MFU readings of 1256 and 509 for SARS-CoV and MERS-CoV N proteins, respectively, while serum 54 has 205 and 1377, respectively. However, in comparison to the MFU readings of positive control sera (ranging from 8127 to 29,319), these low range "potentially positive" readings were considered to be non-conclusive. The clear negative VNT results for these two samples confirmed that they did not contain MERS-CoV antibody.
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