Author: Falzarano, Darryl; de Wit, Emmie; Martellaro, Cynthia; Callison, Julie; Munster, Vincent J.; Feldmann, Heinz
Title: Inhibition of novel ß coronavirus replication by a combination of interferon-a2b and ribavirin Document date: 2013_4_18
ID: tgngoea8_20
Snippet: Western blot. Cell lysates were run on 10% SDS-PAGE gels and transferred to PVDF (GE Healthcare, Piscataway, NJ). Membranes were blocked with 5% non-fat milk, 0.05% Tween20 in PBS and subsequently probed with polyclonal serum diluted in blocking buffer at 1/10,000 from rabbit A691/A741 immunized with inactivated HCoV-EMC. Anti-rabbit IgG conjugated to horseradish peroxidase (KPL, Gaithersburg, MD) was used as a secondary at a 1/10,000 dilution. W.....
Document: Western blot. Cell lysates were run on 10% SDS-PAGE gels and transferred to PVDF (GE Healthcare, Piscataway, NJ). Membranes were blocked with 5% non-fat milk, 0.05% Tween20 in PBS and subsequently probed with polyclonal serum diluted in blocking buffer at 1/10,000 from rabbit A691/A741 immunized with inactivated HCoV-EMC. Anti-rabbit IgG conjugated to horseradish peroxidase (KPL, Gaithersburg, MD) was used as a secondary at a 1/10,000 dilution. Western blots were developed with the Pierce ECL Plus kit (Thermo, Rockford, IL).
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