Author: Baldwin, Don A.; Feldman, Michael; Alwine, James C.; Robertson, Erle S.
Title: Metagenomic Assay for Identification of Microbial Pathogens in Tumor Tissues Document date: 2014_9_16
ID: xlqdn0c7_30
Snippet: Labeled samples were hybridized to microarrays as described in the Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis protocol (version 7.2, G4410-90010). Master mixes containing aCGH blocking agent, HI-RPM hybridization buffer, and Cot-1 DNA (pilot assays only) were added to a mixture of the entire labeled test sample and the xhh DNA control sample, denatured, and hybridized to arrays under 8-chamber gasket slides at 65°C with 20-.....
Document: Labeled samples were hybridized to microarrays as described in the Agilent Oligonucleotide Array-Based CGH for Genomic DNA Analysis protocol (version 7.2, G4410-90010). Master mixes containing aCGH blocking agent, HI-RPM hybridization buffer, and Cot-1 DNA (pilot assays only) were added to a mixture of the entire labeled test sample and the xhh DNA control sample, denatured, and hybridized to arrays under 8-chamber gasket slides at 65°C with 20-rpm rotation for 40 h in an Agilent hybridization oven. Arrays were processed using wash procedure A and scanned on an Agilent SureScan G4900DA microarray scanner.
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