Selected article for: "chase medium and labeling medium"
Title: Milieu-induced, selective aggregation of regulated secretory proteins in the trans-Golgi network
  • Document date: 1991_12_2
    • ID: syyi2ysq_10
    Snippet: [35S]sulfate incorporation into glycosaminoglycan (GAG) chains continued to occur during the first 30 min of chase (see Fig . 4 B) , which probably reflected a relatively slow loss of radioactivity from the PAPS pool . In other experiments, the preincubation in sulfate-free medium, pulse labeling, and chase were performed in air at 20°C instead of 37°C, using DMEM containing 20 mM Hepes-NaOH, pH 7.4, instead of bicarbonate......
    Document: [35S]sulfate incorporation into glycosaminoglycan (GAG) chains continued to occur during the first 30 min of chase (see Fig . 4 B) , which probably reflected a relatively slow loss of radioactivity from the PAPS pool . In other experiments, the preincubation in sulfate-free medium, pulse labeling, and chase were performed in air at 20°C instead of 37°C, using DMEM containing 20 mM Hepes-NaOH, pH 7.4, instead of bicarbonate.

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