Selected article for: "normal concentration and pc12 cell"

Title: Milieu-induced, selective aggregation of regulated secretory proteins in the trans-Golgi network
  • Document date: 1991_12_2
  • ID: syyi2ysq_7
    Snippet: Cell Culture and Metabolic Labeling PC12 cells were grown as previously described (lboze and Hutmer, 1990) . lb label granins present in the RER, PC12 cells were preincubated for 30 min in tyrosine-free DMEM (DMEM without tyrosine and with 10% of the normal concentration of phenylalanine) and then pulse labeled for 5 min with fresh tyrosine-free DMEM containing 100 14Ci/ml L-[2,3,5,6-3H]-.....
    Document: Cell Culture and Metabolic Labeling PC12 cells were grown as previously described (lboze and Hutmer, 1990) . lb label granins present in the RER, PC12 cells were preincubated for 30 min in tyrosine-free DMEM (DMEM without tyrosine and with 10% of the normal concentration of phenylalanine) and then pulse labeled for 5 min with fresh tyrosine-free DMEM containing 100 14Ci/ml L-[2,3,5,6-3H]-

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