Author: Jo, Seri; Kim, Suwon; Shin, Dong Hae; Kim, Mi-Sun
Title: Inhibition of SARS-CoV 3CL protease by flavonoids Document date: 2019_11_14
ID: vynk8q8c_18
Snippet: The three compounds showed the severely reduced fluorescent intensity and thus represented their SARS-CoV 3CLpro inhibitory activity. The IC 50 values were calculated from the dose-dependent inhibitory curves of herbacetin, rhoifolin and pectolinarin. The measured values were 33.17, 27.45 and 37.78 lM, respectively. Since flavonoids are known to be aggregated through complexity and thus non-specifically inhibit various proteases, the assay in the.....
Document: The three compounds showed the severely reduced fluorescent intensity and thus represented their SARS-CoV 3CLpro inhibitory activity. The IC 50 values were calculated from the dose-dependent inhibitory curves of herbacetin, rhoifolin and pectolinarin. The measured values were 33.17, 27.45 and 37.78 lM, respectively. Since flavonoids are known to be aggregated through complexity and thus non-specifically inhibit various proteases, the assay in the presence of Triton X-100 was also performed 24 . Before the examination, we tested the effects of Triton X-100 on the catalytic activity of the SARS-CoV 3CLpro. As shown in Supplementary Figure 1 , only a slight increase in catalyst activity was observed up to 0.1% Triton X-100. Therefore, the assay was performed at a concentration of 0.01% Triton X-100 with no significant interference detected.
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