Author: Bjarnason, Agnar; Westin, Johan; Lindh, Magnus; Andersson, Lars-Magnus; Kristinsson, Karl G; Löve, Arthur; Baldursson, Olafur; Gottfredsson, Magnus
Title: Incidence, Etiology, and Outcomes of Community-Acquired Pneumonia: A Population-Based Study Document date: 2018_2_8
ID: sw8ghj6q_9
Snippet: Oropharyngeal swabs were frozen immediately at −80°C for batch analysis with PCR in 2 steps. Samples were initially tested for seasonal and pandemic influenza, Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila as previously described [19] . Samples were stored at −80°C and reanalyzed with multiplex real-time PCR for S pneumoniae, Haemophilus influenzae, influenza A and B, rhinovirus, adenovirus, parainfluenza 1-3, .....
Document: Oropharyngeal swabs were frozen immediately at −80°C for batch analysis with PCR in 2 steps. Samples were initially tested for seasonal and pandemic influenza, Mycoplasma pneumoniae, Chlamydophila pneumoniae, and Legionella pneumophila as previously described [19] . Samples were stored at −80°C and reanalyzed with multiplex real-time PCR for S pneumoniae, Haemophilus influenzae, influenza A and B, rhinovirus, adenovirus, parainfluenza 1-3, respiratory syncytial virus (RSV), coronavirus (NL63, OC43, HKU1), enterovirus, metapneumovirus, bocavirus, C pneumoniae, and M pneumoniae as previously described [20, 21] . Results of real-time PCR for S pneumoniae and H influenzae were considered positive at a cycle threshold of 35 or less to exclude false-positive results due to carriage [21] .
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