Author: Assiri, Abdullah M.; Biggs, Holly M.; Abedi, Glen R.; Lu, Xiaoyan; Bin Saeed, Abdulaziz; Abdalla, Osman; Mohammed, Mutaz; Al-Abdely, Hail M.; Algarni, Homoud S.; Alhakeem, Raafat F.; Almasri, Malak M.; Alsharef, Ali A.; Nooh, Randa; Erdman, Dean D.; Gerber, Susan I.; Watson, John T.
Title: Increase in Middle East Respiratory Syndrome-Coronavirus Cases in Saudi Arabia Linked to Hospital Outbreak With Continued Circulation of Recombinant Virus, July 1–August 31, 2015 Document date: 2016_8_3
ID: qxk97kdz_8
Snippet: All MERS-CoV cases were confirmed in SA by testing of respiratory specimens by real-time reverse transcriptionpolymerase chain reaction assays targeting regions upstream of the envelope gene (upE) and open reading frame (ORF) 1a [6] . Available specimen aliquots that tested positive for MERS-CoV by both assays during July-August 2015 were shipped to the Centers for Disease Control and Prevention ([CDC] Atlanta, GA) for confirmatory testing and ge.....
Document: All MERS-CoV cases were confirmed in SA by testing of respiratory specimens by real-time reverse transcriptionpolymerase chain reaction assays targeting regions upstream of the envelope gene (upE) and open reading frame (ORF) 1a [6] . Available specimen aliquots that tested positive for MERS-CoV by both assays during July-August 2015 were shipped to the Centers for Disease Control and Prevention ([CDC] Atlanta, GA) for confirmatory testing and genome sequencing. Methodology for sequencing, phylogenetic, and recombinational analyses has been described previously [7] and is available as Supplementary Material. This investigation was part of an urgent public health response and was determined to be nonresearch by the SA MoH and CDC.
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