Selected article for: "cytoplasmic tail and Golgi localization"
Title: Localization and targeting of the Saccharomyces cerevisiae Kre2p/Mnt1p alpha 1,2-mannosyltransferase to a medial-Golgi compartment
  • Document date: 1995_11_2
    • ID: q1jx0n0l_34
    Snippet: The Kre2p cytoplasmic tail NH2 terminus was deleted to further explore its role in targeting. Chimeric construct MR/KKK lacks the Kre2p cytoplasmic tail, with the exception of the initiating methionine codon and the Arg residue lying directly adjacent to the TMD. MR/KKK was Figure 7 . Golgi localization of chimeric proteins KDKK, KPKK, and KD-K by indirect immunofluorescence. Yeast ceils (SEY6210) carrying a KRE2 disruption (kre2::TRP1) and conta.....
    Document: The Kre2p cytoplasmic tail NH2 terminus was deleted to further explore its role in targeting. Chimeric construct MR/KKK lacks the Kre2p cytoplasmic tail, with the exception of the initiating methionine codon and the Arg residue lying directly adjacent to the TMD. MR/KKK was Figure 7 . Golgi localization of chimeric proteins KDKK, KPKK, and KD-K by indirect immunofluorescence. Yeast ceils (SEY6210) carrying a KRE2 disruption (kre2::TRP1) and containing the different chimeric constructions on YEp352 were fixed, spheroplasted, attached to polylysine-coated glass slides, and incubated with anti-Kre2p antibodies followed by a subsequent incubation with a Texas red-conjugated secondary Ab and DAPI.

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