Author: Rathore, Shailendra S.; Liu, Yinghui; Yu, Haijia; Wan, Chun; Lee, MyeongSeon; Yin, Qian; Stowell, Michael H.B.; Shen, Jingshi
Title: Intracellular Vesicle Fusion Requires a Membrane-Destabilizing Peptide Located at the Juxtamembrane Region of the v-SNARE Document date: 2019_12_24
ID: pudp1eoo_40
Snippet: ITC measurements-ITC experiments were performed at 25°C using a VP-ITC instrument (MicroCal). Munc18-1 and SNAREs were dialyzed overnight separately in an ITC binding buffer (25 mM HEPES [pH 7.4], 150 mM KCl, 10% Glycerol, and 0.5 mM TCEP) Yu et al., 2013b) . Munc18-1 protein (5 μM) was loaded into the sample cell of VP-ITC, followed by iterative injection of SNARE complexes (75 μM) into the sample cell. After polynomial baseline correction to.....
Document: ITC measurements-ITC experiments were performed at 25°C using a VP-ITC instrument (MicroCal). Munc18-1 and SNAREs were dialyzed overnight separately in an ITC binding buffer (25 mM HEPES [pH 7.4], 150 mM KCl, 10% Glycerol, and 0.5 mM TCEP) Yu et al., 2013b) . Munc18-1 protein (5 μM) was loaded into the sample cell of VP-ITC, followed by iterative injection of SNARE complexes (75 μM) into the sample cell. After polynomial baseline correction to remove a slight drift of the initial data points, the data were fitted with a nonlinear least-squares routine using the MicroCal Origin software.
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