Title: Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans Document date: 1991_12_2
ID: qrg1rtzi_10
Snippet: Selected XZAP II clones were subcloned into M13 and sequenced by the dideoxy" chain termination method (43) using deoxyinosine triphosphate in place of dGTP and Sequenase (United States Biochemical) as described by the manufacturer. Sequence data were obtained from successive deletions in M13 using T4 polymerase (Cyclone Biosystem, International Biotechnologies Inc., New Haven, CT) or using synthetic oligonucleotide primers. Oligonucleotides were.....
Document: Selected XZAP II clones were subcloned into M13 and sequenced by the dideoxy" chain termination method (43) using deoxyinosine triphosphate in place of dGTP and Sequenase (United States Biochemical) as described by the manufacturer. Sequence data were obtained from successive deletions in M13 using T4 polymerase (Cyclone Biosystem, International Biotechnologies Inc., New Haven, CT) or using synthetic oligonucleotide primers. Oligonucleotides were synthesized on an Applied Biosystems (model 380B) DNA synthesizer.
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