Title: Isolation, characterization, and expression of cDNAs encoding murine alpha-mannosidase II, a Golgi enzyme that controls conversion of high mannose to complex N-glycans Document date: 1991_12_2
ID: qrg1rtzi_6
Snippet: Man II was assayed using 4-methylumbelliferyl a-D-mannoside as substrate as described (27) . NaCl-washed microsomal membranes from COS cell monolayers were prepared from four 100-mm culture dishes (90% confluent) exactly as previously described for 3T3 cells (27) . Protein concentration was determined using the BCA protein assay reagent as described by Pierce Chemical Co. (Rockford, IL) using BSA as standard......
Document: Man II was assayed using 4-methylumbelliferyl a-D-mannoside as substrate as described (27) . NaCl-washed microsomal membranes from COS cell monolayers were prepared from four 100-mm culture dishes (90% confluent) exactly as previously described for 3T3 cells (27) . Protein concentration was determined using the BCA protein assay reagent as described by Pierce Chemical Co. (Rockford, IL) using BSA as standard.
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