Author: Pérez-Ruiz, Mercedes; Pedrosa-Corral, Irene; Sanbonmatsu-Gámez, Sara; Navarro-Marí, José-María
Title: Laboratory Detection of Respiratory Viruses by Automated Techniques Document date: 2012_11_30
ID: ted64zo4_57
Snippet: The FilmArray ® Respiratory Panel (Idaho Technology Inc.) is commercialised in Europe to fully automate the detection and identification of 21 respiratory pathogens from a single sample in about one hour, including 18 viruses and 3 bacteria. An unprocessed clinical sample is subjected to nucleic acid purification, RT, a high-order nested multiplex PCR and amplicon melting curve analysis in a closed disposable pouch [51] . The larger spectrum of .....
Document: The FilmArray ® Respiratory Panel (Idaho Technology Inc.) is commercialised in Europe to fully automate the detection and identification of 21 respiratory pathogens from a single sample in about one hour, including 18 viruses and 3 bacteria. An unprocessed clinical sample is subjected to nucleic acid purification, RT, a high-order nested multiplex PCR and amplicon melting curve analysis in a closed disposable pouch [51] . The larger spectrum of this panel that allows the additional detection of other respiratory pathogens may be an advantage for diagnosing ARI in some population groups [52] . However, detection of more pathogens leads to a more expensive system. This assay is probably costeffective for rapid detection of respiratory agents from certain clinically severe patients. Table 2 displays a list of commercial fully-automated assays for detection and identification of RV.
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