Author: Grellet, A.; Heilmann, R.M.; Polack, B.; Feugier, A.; Boucraut-Baralon, C.; Grandjean, D.; Grützner, N.; Suchodolski, J. S.; Steiner, J.M.; Chastant-Maillard, S.
Title: Influence of Breed Size, Age, Fecal Quality, and Enteropathogen Shedding on Fecal Calprotectin and Immunoglobulin A Concentrations in Puppies During the Weaning Period Document date: 2016_6_8
ID: yxwvchvu_12
Snippet: Feces were evaluated for the presence of DNA and RNA from CPV2 and CCV by qPCR and qRT-PCR, respectively, as previously described. 6 Results from duplicate PCR analyses from the extracted DNA (ie, 2 PCR assay were performed for each fecal extract) were expressed semiquantitatively as virus loads. Puppies were defined as infected by CPV2 and CCV if viral loads were >10 10.3 and 10 9.3 copies respectively. 6 After extraction, calprotectin and IgA w.....
Document: Feces were evaluated for the presence of DNA and RNA from CPV2 and CCV by qPCR and qRT-PCR, respectively, as previously described. 6 Results from duplicate PCR analyses from the extracted DNA (ie, 2 PCR assay were performed for each fecal extract) were expressed semiquantitatively as virus loads. Puppies were defined as infected by CPV2 and CCV if viral loads were >10 10.3 and 10 9.3 copies respectively. 6 After extraction, calprotectin and IgA were quantified by inhouse radioimmunoassays as previously described. 22, 32, 34 All samples were analyzed using the same batch of tracer and reagents.
Search related documents:
Co phrase search for related documents- extract dna and PCR assay: 1, 2, 3
- fecal extract and PCR analysis: 1
- PCR analysis and qpcr qrt: 1, 2
- PCR analysis and viral load: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- PCR analysis and virus load: 1
- PCR assay and qpcr qrt: 1
- PCR assay and RNA DNA presence: 1
- PCR assay and viral load: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- PCR assay and virus load: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11
- previously describe and virus load: 1
- RNA DNA presence and viral load: 1
- viral load and virus load: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
Co phrase search for related documents, hyperlinks ordered by date