Author: Baldwin, Don A.; Feldman, Michael; Alwine, James C.; Robertson, Erle S.
Title: Metagenomic Assay for Identification of Microbial Pathogens in Tumor Tissues Document date: 2014_9_16
ID: xlqdn0c7_9
Snippet: Pilot experiment 1 indicated that the presence or absence of Cot-1 DNA made no difference in probe performance (Tables 1 and 2 ). Therefore, this reagent was omitted from subsequent assays. Interestingly, very high hybridization intensities were noted for probes to Epstein-Barr virus (EBV; human herpesvirus 4) from this control human DNA. The manufacturer confirmed that cell lines used to prepare the male and female SureTag human reference DNA w.....
Document: Pilot experiment 1 indicated that the presence or absence of Cot-1 DNA made no difference in probe performance (Tables 1 and 2 ). Therefore, this reagent was omitted from subsequent assays. Interestingly, very high hybridization intensities were noted for probes to Epstein-Barr virus (EBV; human herpesvirus 4) from this control human DNA. The manufacturer confirmed that cell lines used to prepare the male and female SureTag human reference DNA were infected with this virus to generate the cell lines. EBV detection in tumor screening assays that used this reagent was therefore possible if the signals were not normalized to EBV probe signals to the xhh Cy5 channel. Future assays for our screens utilized virus-free reference human DNA as the crosshybridization control after validation through a number of stringent steps for detection of other known viruses.
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