Author: Dejnirattisai, Wanwisa; Webb, Andrew I.; Chan, Vera; Jumnainsong, Amonrat; Davidson, Andrew; Mongkolsapaya, Juthathip; Screaton, Gavin
Title: Lectin Switching During Dengue Virus Infection Document date: 2011_6_15
ID: qos9vu3r_20
Snippet: Following the bite from an infected mosquito, the host first encounters virus produced in the mosquito, and following this initial inoculation subsequent rounds of infection are driven by virus produced by host cells. To study these 2 distinct stages of pathogenesis, we compared viruses from C6/36 (insect cells) and virus produced from primary human monocyte-derived dendritic cells. Viral supernatants were titered using a focus-forming assay on V.....
Document: Following the bite from an infected mosquito, the host first encounters virus produced in the mosquito, and following this initial inoculation subsequent rounds of infection are driven by virus produced by host cells. To study these 2 distinct stages of pathogenesis, we compared viruses from C6/36 (insect cells) and virus produced from primary human monocyte-derived dendritic cells. Viral supernatants were titered using a focus-forming assay on Vero cells, and equal amounts of titered virus were used to infect Vero, 293T, or DCs at an MOI of 1. The percentage of infected cells was monitored by cytofluorometry staining of the intracellular nonstructural dengue antigen NS1, which is produced only following productive infection. Insect-derived virus was equally competent at infecting the 3 cell types with high efficiency ( Figure 1A-C) . Surprisingly, DC-produced virus was not able to reinfect DCs but was nevertheless fully competent at infecting 293T and Vero cells.
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