Author: Dejnirattisai, Wanwisa; Webb, Andrew I.; Chan, Vera; Jumnainsong, Amonrat; Davidson, Andrew; Mongkolsapaya, Juthathip; Screaton, Gavin
Title: Lectin Switching During Dengue Virus Infection Document date: 2011_6_15
ID: qos9vu3r_30
Snippet: Finally, we assessed the N-linked glycan on the envelope protein by digestion with either Endo H, which will remove high-mannose simple glycans containing 3 or more terminal mannose residues, or PNGase F, which removes all simple or complex N-linked glycan ( Figure 4B ). DC-produced virus showed a mobility shift of around 3 or 6 kDa corresponding to the addition of 1 or 2 complex carbohydrates at positions 67 and 153. This was completely resistan.....
Document: Finally, we assessed the N-linked glycan on the envelope protein by digestion with either Endo H, which will remove high-mannose simple glycans containing 3 or more terminal mannose residues, or PNGase F, which removes all simple or complex N-linked glycan ( Figure 4B ). DC-produced virus showed a mobility shift of around 3 or 6 kDa corresponding to the addition of 1 or 2 complex carbohydrates at positions 67 and 153. This was completely resistant to digestion with Endo H, indicating the presence of complex low-mannose carbohydrate.
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