Selected article for: "extraction reagent and RNA extraction reagent"

Author: He, Yang; Cao, Lingling; Wang, Li; Liu, Lingping; Huang, Ying; Gong, Xuan
Title: Metformin Inhibits Proliferation of Human Thyroid Cancer TPC-1 Cells by Decreasing LRP2 to Suppress the JNK Pathway
  • Document date: 2020_1_6
  • ID: uzatktbf_11
    Snippet: Extraction of total RNA in cells was performed using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and RNA was subjected to reverse transcription. The extracted cDNA was applied for PCR using SYBR Green method (TaKaRa, Tokyo, Japan). Primer sequences were as follows: LRP2: F: 5′-GATCTGTGACCTTCATTCCTGGCCTGATC-3′, R: 5′-GCCATGACACCTGTAGATGTGGTGCTGAATAATTG-GTTAA-3′; Glyceraldehyde 3-phosphate dehydrogenase (GAPDH): F: 5′-ACTGCCACCCAGAAGAC.....
    Document: Extraction of total RNA in cells was performed using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and RNA was subjected to reverse transcription. The extracted cDNA was applied for PCR using SYBR Green method (TaKaRa, Tokyo, Japan). Primer sequences were as follows: LRP2: F: 5′-GATCTGTGACCTTCATTCCTGGCCTGATC-3′, R: 5′-GCCATGACACCTGTAGATGTGGTGCTGAATAATTG-GTTAA-3′; Glyceraldehyde 3-phosphate dehydrogenase (GAPDH): F: 5′-ACTGCCACCCAGAAGACT-3′, R: 5ʹ-GCTCAGTGTAGCCCAGGAT-3′.

    Search related documents:
    Co phrase search for related documents
    • cell total RNA extraction and transcription reverse: 1
    • PCR SYBR Green method and SYBR Green method: 1, 2, 3, 4, 5, 6
    • PCR SYBR Green method and total RNA extraction: 1
    • PCR SYBR Green method and transcription reverse: 1, 2
    • Primer sequence and total RNA extraction: 1, 2
    • Primer sequence and transcription reverse: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21
    • SYBR Green method and total RNA extraction: 1
    • SYBR Green method and transcription reverse: 1, 2, 3, 4
    • total RNA extraction and transcription reverse: 1, 2, 3, 4, 5, 6, 7, 8