Author: Imai-Matsushima, Aki; Martin-Sancho, Laura; Karlas, Alexander; Imai, Seiichiro; Zoranovic, Tamara; Hocke, Andreas C.; Mollenkopf, Hans-Joachim; Berger, Hilmar; Meyer, Thomas F.
Title: Long-Term Culture of Distal Airway Epithelial Cells Allows Differentiation Towards Alveolar Epithelial Cells Suited for Influenza Virus Studies Document date: 2018_6_22
ID: vopbw1tl_23
Snippet: Removal of feeders and transfer to a conventional culture environment did not induce differentiation into mature alveolar epithelial cells, as qRT-PCR analyses revealed that the AEI cell marker AQP5 was downregulated 6 days later (Fig. 3A) . Instead, SCGB1A1 was upregulated, suggesting differentiation toward an airway club cell phenotype (Fig. 3B ). Immunofluorescence analysis at 72 h confirmed the presence of SCGB1A1 (Fig. 3C ). Microarray analy.....
Document: Removal of feeders and transfer to a conventional culture environment did not induce differentiation into mature alveolar epithelial cells, as qRT-PCR analyses revealed that the AEI cell marker AQP5 was downregulated 6 days later (Fig. 3A) . Instead, SCGB1A1 was upregulated, suggesting differentiation toward an airway club cell phenotype (Fig. 3B ). Immunofluorescence analysis at 72 h confirmed the presence of SCGB1A1 (Fig. 3C ). Microarray analyses showed strong up-regulation of inflammatory cytokines, tissue remodeling-associated proteases, protease inhibitors, collagen 1A1 as well as SCGB1A1 (Fig. 3D ). In contrast, RASSF2, a component of the NF-κB pathway, was downregulated.
Search related documents:
Co phrase search for related documents- alveolar epithelial cell and cell phenotype: 1
- alveolar epithelial cell and epithelial cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- alveolar epithelial cell and feeder removal: 1
- alveolar epithelial cell and immunofluorescence analysis: 1
- alveolar epithelial cell and inflammatory cytokine: 1
- cell marker and differentiation induce: 1, 2
- cell marker and epithelial cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
- cell marker and feeder removal: 1
- cell marker and immunofluorescence analysis: 1, 2, 3
- cell marker and inflammatory cytokine: 1, 2, 3, 4, 5, 6
- cell marker and microarray analysis: 1
- cell phenotype and club cell phenotype: 1
- cell phenotype and culture environment: 1, 2
- cell phenotype and epithelial cell: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
- cell phenotype and feeder removal: 1
- cell phenotype and inflammatory cytokine: 1, 2, 3, 4, 5, 6, 7
- cell phenotype and PCR analysis: 1
- cell phenotype and protease inhibitor: 1
- club cell phenotype and feeder removal: 1
Co phrase search for related documents, hyperlinks ordered by date