Author: Imai-Matsushima, Aki; Martin-Sancho, Laura; Karlas, Alexander; Imai, Seiichiro; Zoranovic, Tamara; Hocke, Andreas C.; Mollenkopf, Hans-Joachim; Berger, Hilmar; Meyer, Thomas F.
Title: Long-Term Culture of Distal Airway Epithelial Cells Allows Differentiation Towards Alveolar Epithelial Cells Suited for Influenza Virus Studies Document date: 2018_6_22
ID: vopbw1tl_23
Snippet: Removal of feeders and transfer to a conventional culture environment did not induce differentiation into mature alveolar epithelial cells, as qRT-PCR analyses revealed that the AEI cell marker AQP5 was downregulated 6 days later (Fig. 3A) . Instead, SCGB1A1 was upregulated, suggesting differentiation toward an airway club cell phenotype (Fig. 3B ). Immunofluorescence analysis at 72 h confirmed the presence of SCGB1A1 (Fig. 3C ). Microarray analy.....
Document: Removal of feeders and transfer to a conventional culture environment did not induce differentiation into mature alveolar epithelial cells, as qRT-PCR analyses revealed that the AEI cell marker AQP5 was downregulated 6 days later (Fig. 3A) . Instead, SCGB1A1 was upregulated, suggesting differentiation toward an airway club cell phenotype (Fig. 3B ). Immunofluorescence analysis at 72 h confirmed the presence of SCGB1A1 (Fig. 3C ). Microarray analyses showed strong up-regulation of inflammatory cytokines, tissue remodeling-associated proteases, protease inhibitors, collagen 1A1 as well as SCGB1A1 (Fig. 3D ). In contrast, RASSF2, a component of the NF-κB pathway, was downregulated.
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