Selected article for: "PCR product and sequence plasmid"
Title: Localization of TGN38 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing sequence
  • Document date: 1993_3_1
    • ID: qt44izzh_7
    Snippet: A DNA fragment encoding the transmembrane and cytoplasmic domains of TGN38 (Luzio et al., 1990) was cloned from rat liver RNA by RNA-PCR, performed according to the manufacturer's guidelines (Perkin-Elmer Cetus, Norwalk, CT). The PCR product was cloned into a BgllI-XbaI cut pCDM8-'lhc plasmid, containing a sequence encoding the Tac lumenal domain (Rutledge et al., 1992) . All subsequent Tac-TGN38 chimeric and mu-tant DNAs were constructed by PCR,.....
    Document: A DNA fragment encoding the transmembrane and cytoplasmic domains of TGN38 (Luzio et al., 1990) was cloned from rat liver RNA by RNA-PCR, performed according to the manufacturer's guidelines (Perkin-Elmer Cetus, Norwalk, CT). The PCR product was cloned into a BgllI-XbaI cut pCDM8-'lhc plasmid, containing a sequence encoding the Tac lumenal domain (Rutledge et al., 1992) . All subsequent Tac-TGN38 chimeric and mu-tant DNAs were constructed by PCR, according to the procedure of Higuchi et al. (1988) and also cloned into pCDMS. The sequence of all the recombinant constructs was confirmed by the dideoxy chain-termination method.

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