Author: Ding, Peiyang; Zhang, Teng; Li, Yafei; Teng, Man; Sun, Yaning; Liu, Xiao; Chai, Shujun; Zhou, Enmin; Jin, Qianyue; Zhang, Gaiping
Title: Nanoparticle orientationally displayed antigen epitopes improve neutralizing antibody level in a model of porcine circovirus type 2 Document date: 2017_7_24
ID: upb97on4_17
Snippet: Cap-AuNP cytotoxicity was evaluated using the cellcounting kit 8 (CCK-8, Beyotime, Wuhan, People's Republic of China). 31 RAW264.7 and DC2.4 cells were seeded at 5×10 3 cells/well on 96-well cell culture plates, respectively. After cell adherence, different concentrations of Cap-AuNPs were added to incubate with the cells for 24 h. Then the medium was removed before adding 100 μL fresh medium containing 10% CCK-8 reagent. The culture plates wer.....
Document: Cap-AuNP cytotoxicity was evaluated using the cellcounting kit 8 (CCK-8, Beyotime, Wuhan, People's Republic of China). 31 RAW264.7 and DC2.4 cells were seeded at 5×10 3 cells/well on 96-well cell culture plates, respectively. After cell adherence, different concentrations of Cap-AuNPs were added to incubate with the cells for 24 h. Then the medium was removed before adding 100 μL fresh medium containing 10% CCK-8 reagent. The culture plates were incubated for another 4 h. After incubation, the absorbance at 450 nm was measured using a microplate reader.
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