Author: Chu, Daniel K. W.; Hui, Kenrie P. Y.; Perera, Ranawaka A. P. M.; Miguel, Eve; Niemeyer, Daniela; Zhao, Jincun; Channappanavar, Rudragouda; Dudas, Gytis; Oladipo, Jamiu O.; Traoré, Amadou; Fassi-Fihri, Ouafaa; Ali, Abraham; Demissié, Getnet F.; Muth, Doreen; Chan, Michael C. W.; Nicholls, John M.; Meyerholz, David K.; Kuranga, Sulyman A.; Mamo, Gezahegne; Zhou, Ziqi; So, Ray T. Y.; Hemida, Maged G.; Webby, Richard J.; Roger, Francois; Rambaut, Andrew; Poon, Leo L. M.; Perlman, Stanley; Drosten, Christian; Chevalier, Veronique; Peiris, Malik
Title: MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity Document date: 2018_3_20
ID: riitjx0f_28
Snippet: Phylogenetic Analysis and Phenotypic Comparison of MERS-CoV Isolates. The phylogenetic analysis shown in Fig. 1 was done using the maximum-likelihood method (PhyML) (24) . Other phylogenetic methods are detailed in Supporting Information. Dromedary virus BF785 and Nig1657 viruses isolated as part of this study were chosen for comparison with the prototype human MERS-CoV strain EMC (clade A) provided by R. A. M. Fouchier (Erasmus University Medica.....
Document: Phylogenetic Analysis and Phenotypic Comparison of MERS-CoV Isolates. The phylogenetic analysis shown in Fig. 1 was done using the maximum-likelihood method (PhyML) (24) . Other phylogenetic methods are detailed in Supporting Information. Dromedary virus BF785 and Nig1657 viruses isolated as part of this study were chosen for comparison with the prototype human MERS-CoV strain EMC (clade A) provided by R. A. M. Fouchier (Erasmus University Medical Center, Rotterdam, The Netherlands) and a dromedary camel virus, dromedary/Al-Hasa-KFU-HKU13/2013 (AH13) (clade B), isolated in the Kingdom of Saudi Arabia (25) . Viruses were isolated and grown in Vero cells, aliquoted, and stored at −80°C until used. The passage history of these viruses is shown in Dataset S1. Antigenic comparison also included dromedary/Egypt NRCE-HKU-270 (15) .
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