Author: Ma, J; Wan, J; Meng, J; Banerjee, S; Ramakrishnan, S; Roy, S
Title: Methamphetamine induces autophagy as a pro-survival response against apoptotic endothelial cell death through the Kappa opioid receptor Document date: 2014_3_6
ID: wu2mogfa_27
Snippet: Real-time RT-PCR. Total RNA isolated from HUVECs was subjected to twostep reverse transcription-PCR (RT-PCR). Real-time SYBR green PCR analysis was performed for Beclin1 (sense, 5 0 -GCTGAGAGACTGGATCAGGA-3 0 ; antisense, 5 0 -ATTGTGCCAAACTGTCCACT-3 0 ) and human KOR(sense, 5 0 -GAAACAAGCC CTGGTGAAAT-3 0 ; antisense, 5 0 -TGAGCCAAGACTGTGCTACC-3 0 ). Relative quantification was performed using the ABI Prism 7500 real-time PCR system (Applied Biosys.....
Document: Real-time RT-PCR. Total RNA isolated from HUVECs was subjected to twostep reverse transcription-PCR (RT-PCR). Real-time SYBR green PCR analysis was performed for Beclin1 (sense, 5 0 -GCTGAGAGACTGGATCAGGA-3 0 ; antisense, 5 0 -ATTGTGCCAAACTGTCCACT-3 0 ) and human KOR(sense, 5 0 -GAAACAAGCC CTGGTGAAAT-3 0 ; antisense, 5 0 -TGAGCCAAGACTGTGCTACC-3 0 ). Relative quantification was performed using the ABI Prism 7500 real-time PCR system (Applied Biosystems, Grand Island, NY, USA). Beclin1 transcript levels were normalized to b-actin (sense, 5 0 -GGACTTCGAGCAAGAGATGG-3 0 ; antisense, 5 0 -AGCACTGTG TTG GCGTACAG-3 0 ) transcript levels from the same preparations of cDNA.
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