Author: Ma, J; Wan, J; Meng, J; Banerjee, S; Ramakrishnan, S; Roy, S
Title: Methamphetamine induces autophagy as a pro-survival response against apoptotic endothelial cell death through the Kappa opioid receptor Document date: 2014_3_6
ID: wu2mogfa_4
Snippet: METH inhibits endothelial cell viability and disrupts cellcell barrier integrity. We initially determined if METH treatment reduced cell viability. The blood concentrations of METH in drug abusers are in the low micromolar range, with a median concentration of 1.25 mM. 10 Therefore, human umbilical vein endothelial cells (HUVECs) were exposed to 1 mM METH for 24, 48 or 72 h and cell viability determined using a MTT assay. Although, no obvious cha.....
Document: METH inhibits endothelial cell viability and disrupts cellcell barrier integrity. We initially determined if METH treatment reduced cell viability. The blood concentrations of METH in drug abusers are in the low micromolar range, with a median concentration of 1.25 mM. 10 Therefore, human umbilical vein endothelial cells (HUVECs) were exposed to 1 mM METH for 24, 48 or 72 h and cell viability determined using a MTT assay. Although, no obvious change in viability was observed at 24 and 48 h with low concentration (1 mM) of METH, significant decrease in viability was observed at 72 h compared with control ( Figure 1a ).
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