Author: Kent, A. C. C.; Cross, G.; Taylor, D. R.; Sherwood, R. A.; Watson, P. J.
Title: Measurement of serum 7a-hydroxy-4-cholesten-3-one as a marker of bile acid malabsorption in dogs with chronic diarrhoea: a pilot study Document date: 2016_4_6
ID: rkdfde07_9
Snippet: Serum C4 was measured using liquid chromatographymass spectrometry (LC-MS/MS) (see online supplementary appendix 1). Briefly, to 250 μl of the sample was added 250 μl of deuterated internal standard working solution (containing 125 nmol/l of C4-d7 in acetonitrile) followed by a further 500 μl of ice-cold acetonitrile in a tube. Samples were then vortexed for 30 seconds followed by centrifugation for 10 minutes at 16,000g. The supernatant of th.....
Document: Serum C4 was measured using liquid chromatographymass spectrometry (LC-MS/MS) (see online supplementary appendix 1). Briefly, to 250 μl of the sample was added 250 μl of deuterated internal standard working solution (containing 125 nmol/l of C4-d7 in acetonitrile) followed by a further 500 μl of ice-cold acetonitrile in a tube. Samples were then vortexed for 30 seconds followed by centrifugation for 10 minutes at 16,000g. The supernatant of this is transferred to a glass high-performance liquid chromatography injection phial and 50 μl in injected onto the LC-MS/MS system.
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