Selected article for: "Dromedary camel and MERS cov"
Author: Chu, Daniel K. W.; Hui, Kenrie P. Y.; Perera, Ranawaka A. P. M.; Miguel, Eve; Niemeyer, Daniela; Zhao, Jincun; Channappanavar, Rudragouda; Dudas, Gytis; Oladipo, Jamiu O.; Traoré, Amadou; Fassi-Fihri, Ouafaa; Ali, Abraham; Demissié, Getnet F.; Muth, Doreen; Chan, Michael C. W.; Nicholls, John M.; Meyerholz, David K.; Kuranga, Sulyman A.; Mamo, Gezahegne; Zhou, Ziqi; So, Ray T. Y.; Hemida, Maged G.; Webby, Richard J.; Roger, Francois; Rambaut, Andrew; Poon, Leo L. M.; Perlman, Stanley; Drosten, Christian; Chevalier, Veronique; Peiris, Malik
Title: MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity
  • Document date: 2018_3_20
    • ID: riitjx0f_10
    Snippet: The antigenic similarity of BF785 and Nig1657 was compared with prototype strain EMC, AH13, and Egypt NRCE-HKU270 by microneutralization tests using serum from a dromedary camel immunized with a vaccinia-vectored MERS-CoV strain EMC spike vaccine (provided by B. L. Haagmans, Erasmus University Medical Center, Rotterdam) (13) and two fieldcollected dromedary camel sera from Saudi Arabia (Table S1) . Each serum neutralized all MERS-CoV to titer dil.....
    Document: The antigenic similarity of BF785 and Nig1657 was compared with prototype strain EMC, AH13, and Egypt NRCE-HKU270 by microneutralization tests using serum from a dromedary camel immunized with a vaccinia-vectored MERS-CoV strain EMC spike vaccine (provided by B. L. Haagmans, Erasmus University Medical Center, Rotterdam) (13) and two fieldcollected dromedary camel sera from Saudi Arabia (Table S1) . Each serum neutralized all MERS-CoV to titer dilutions twofold or less than observed with prototype virus EMC. . A representative dromedary virus, BF785, with ORF4b deletion type i/v, the most extensive deletion of ORF4b without any ORF3 indel, and Nig1657, with partial deletion of ORF4b (type i/ii) together with an ORF3 deletion, were selected for detailed phenotypic characterization. We confirmed that the limited passage of virus isolates in cell culture had not extended the ORF deletions observed in direct sequences from the specimen. These viruses were compared with the prototype MERS-CoV EMC strain (clade A) and a dromedary virus isolated in the Kingdom of Saudi Arabia AH13 (clade B). Neither EMC nor AH13 has truncations in ORF4b or ORF3.

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