Modulation of nasopharyngeal innate defenses by viral coinfection predisposes individuals to experimental pneumococcal carriage

Glennie, S; Gritzfeld, J F; Pennington, S H; Garner-Jones, M; Coombes, N; Hopkins, M J; Vadesilho, C F; Miyaji, E N; Wang, D; Wright, A D; Collins, A M; Gordon, S B; Ferreira, D M

We were unable to detect FH expression in epithelial cells after in vitro stimulation of nasal wash samples containing virus (data not shown). Previous transcriptome of epithelial cells Figure 5 Pneumococcal surface protein C (PspC) epitope mapping using sera of healthy adults exposed to pneumococcus reveals the lack of antibodies to the Factor H (FH) binding site. The amino acid sequence of PspC group 3 variant was spotted on peptide arrays and is represented on the x axis. Peptide arrays covering the N-terminal region until the proline-rich region were probed with 29 samples from 18 subjects. The number of samples that recognized each peptide is represented on the y axis. Sequences of the peptides recognized by human sera are in black and the ones not recognized are in gray. Binding sites for FH and secretory immunoglobulin A (sIgA) are represented in dashed black and continuous gray boxes, respectively.