Author: Chu, Daniel K. W.; Hui, Kenrie P. Y.; Perera, Ranawaka A. P. M.; Miguel, Eve; Niemeyer, Daniela; Zhao, Jincun; Channappanavar, Rudragouda; Dudas, Gytis; Oladipo, Jamiu O.; Traoré, Amadou; Fassi-Fihri, Ouafaa; Ali, Abraham; Demissié, Getnet F.; Muth, Doreen; Chan, Michael C. W.; Nicholls, John M.; Meyerholz, David K.; Kuranga, Sulyman A.; Mamo, Gezahegne; Zhou, Ziqi; So, Ray T. Y.; Hemida, Maged G.; Webby, Richard J.; Roger, Francois; Rambaut, Andrew; Poon, Leo L. M.; Perlman, Stanley; Drosten, Christian; Chevalier, Veronique; Peiris, Malik
Title: MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity Document date: 2018_3_20
ID: riitjx0f_27
Snippet: Details of the study sites (6, 7) , specimen collection, and RT-PCR detection of MERS RNA are detailed in Supporting Information. Representative swab specimens with high MERS-CoV viral load [low threshold cycle (CT) values] were selected for full-length virus genome sequencing, and additional specimens were selected for targeted sequencing of the ORF3 and ORF4b regions (Supporting Information). Methods used for virus isolation and for the assembl.....
Document: Details of the study sites (6, 7) , specimen collection, and RT-PCR detection of MERS RNA are detailed in Supporting Information. Representative swab specimens with high MERS-CoV viral load [low threshold cycle (CT) values] were selected for full-length virus genome sequencing, and additional specimens were selected for targeted sequencing of the ORF3 and ORF4b regions (Supporting Information). Methods used for virus isolation and for the assembly and modification of a full-length BAC cDNA clone to rescue wild-type and mutant recombinant MERS-CoV lacking ORF4b are described in Supporting Information.
Search related documents:
Co phrase search for related documents- mutant recombinant wild type and viral load: 1
- mutant recombinant wild type and wild type: 1, 2, 3, 4, 5, 6, 7
- RT PCR detection and specimen collection: 1, 2, 3, 4, 5, 6, 7
- RT PCR detection and study site: 1, 2
- RT PCR detection and threshold cycle: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55
- RT PCR detection and viral load: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54
- RT PCR detection and virus genome sequencing: 1
- RT PCR detection and virus isolation: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36
- RT PCR detection and wild type: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13
- specimen collection and study site: 1, 2
- specimen collection and threshold cycle: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
- specimen collection and viral load: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16
- specimen collection and virus genome sequencing: 1
- study site and threshold cycle: 1, 2, 3, 4
- study site and viral load: 1, 2, 3, 4, 5, 6, 7, 8, 9
- study site and wild type: 1, 2, 3, 4, 5, 6, 7
- target sequencing and viral load: 1, 2
- target sequencing and virus genome sequencing: 1, 2, 3
- threshold cycle and viral load: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74
Co phrase search for related documents, hyperlinks ordered by date