Selected article for: "cDNA clone and length clone"
Author: Chu, Daniel K. W.; Hui, Kenrie P. Y.; Perera, Ranawaka A. P. M.; Miguel, Eve; Niemeyer, Daniela; Zhao, Jincun; Channappanavar, Rudragouda; Dudas, Gytis; Oladipo, Jamiu O.; Traoré, Amadou; Fassi-Fihri, Ouafaa; Ali, Abraham; Demissié, Getnet F.; Muth, Doreen; Chan, Michael C. W.; Nicholls, John M.; Meyerholz, David K.; Kuranga, Sulyman A.; Mamo, Gezahegne; Zhou, Ziqi; So, Ray T. Y.; Hemida, Maged G.; Webby, Richard J.; Roger, Francois; Rambaut, Andrew; Poon, Leo L. M.; Perlman, Stanley; Drosten, Christian; Chevalier, Veronique; Peiris, Malik
Title: MERS coronaviruses from camels in Africa exhibit region-dependent genetic diversity
  • Document date: 2018_3_20
    • ID: riitjx0f_27
    Snippet: Details of the study sites (6, 7) , specimen collection, and RT-PCR detection of MERS RNA are detailed in Supporting Information. Representative swab specimens with high MERS-CoV viral load [low threshold cycle (CT) values] were selected for full-length virus genome sequencing, and additional specimens were selected for targeted sequencing of the ORF3 and ORF4b regions (Supporting Information). Methods used for virus isolation and for the assembl.....
    Document: Details of the study sites (6, 7) , specimen collection, and RT-PCR detection of MERS RNA are detailed in Supporting Information. Representative swab specimens with high MERS-CoV viral load [low threshold cycle (CT) values] were selected for full-length virus genome sequencing, and additional specimens were selected for targeted sequencing of the ORF3 and ORF4b regions (Supporting Information). Methods used for virus isolation and for the assembly and modification of a full-length BAC cDNA clone to rescue wild-type and mutant recombinant MERS-CoV lacking ORF4b are described in Supporting Information.

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