Author: Dejnirattisai, Wanwisa; Webb, Andrew I.; Chan, Vera; Jumnainsong, Amonrat; Davidson, Andrew; Mongkolsapaya, Juthathip; Screaton, Gavin
Title: Lectin Switching During Dengue Virus Infection Document date: 2011_6_15
ID: qos9vu3r_17
Snippet: DENV supernatant was precleared with protein A-agarose for one h at 4°C. Bead-free supernatant was incubated with 10 lg of 4G2 at 4°C for 2 h followed by protein A-agarose for 1 h. The beads were washed with .05% Tween/PBS 3 times and eluted with nonreducing loading buffer. The sample was run on nonreducing 10% Sodium dodecyl sulfate (SDS) polyacryramide gels and electroblotted onto nitrocellulose membrane (Amersham). Glycan types on DENV prote.....
Document: DENV supernatant was precleared with protein A-agarose for one h at 4°C. Bead-free supernatant was incubated with 10 lg of 4G2 at 4°C for 2 h followed by protein A-agarose for 1 h. The beads were washed with .05% Tween/PBS 3 times and eluted with nonreducing loading buffer. The sample was run on nonreducing 10% Sodium dodecyl sulfate (SDS) polyacryramide gels and electroblotted onto nitrocellulose membrane (Amersham). Glycan types on DENV proteins were determined using the DIG Glycan Differentiation Kit (Roche).
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