Selected article for: "avian infectious bronchitis virus and bronchitis virus"
Author: Chow, Ken Yan Ching; Hon, Chung Chau; Hui, Raymond Kin Hi; Wong, Raymond Tsz Yeung; Yip, Chi Wai; Zeng, Fanya; Leung, Frederick Chi Ching
Title: Molecular Advances in Severe Acute Respiratory Syndrome-associated Coronavirus (SARS-CoV)
  • Document date: 2016_11_28
    • ID: xuj4yymz_8
    Snippet: The 5 UTR of the SARS-CoV genome was characterized by 5 Rapid Amplification of cDNA Ends (5 RACE; ref. 14 ) and Northern blot assay (13, 16, 22 ) . These procedures elucidated the leader sequence and the transcription regulatory sequence (TRS). The leader sequence found in the viral sg mRNA tran-scripts is at least 72 nucleotides long. Through the alignment of the leader sequence at the 5 end of the eight sg mRNAs, there is a minimal consensus TR.....
    Document: The 5 UTR of the SARS-CoV genome was characterized by 5 Rapid Amplification of cDNA Ends (5 RACE; ref. 14 ) and Northern blot assay (13, 16, 22 ) . These procedures elucidated the leader sequence and the transcription regulatory sequence (TRS). The leader sequence found in the viral sg mRNA tran-scripts is at least 72 nucleotides long. Through the alignment of the leader sequence at the 5 end of the eight sg mRNAs, there is a minimal consensus TRS, namely, 5 -ACGAAC-3 , which participates in the discontinuous synthesis of sg mRNAs as a signaling sequence. The degree of sequence variance flanking the TRS showed no clear relationship with the abundance of the sg mRNAs (22 ) . A highly conserved s2m motif with 32 nucleotides was also identified in the 3 region of the genome, which had also been described in avian infectious bronchitis virus (AIBV; ref. 12 -14 ) .

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