Title: Milieu-induced, selective aggregation of regulated secretory proteins in the trans-Golgi network Document date: 1991_12_2
ID: syyi2ysq_32
Snippet: The vast majority of the [35 S]sulfate-labeled granins were released into the supernatant when TGN vesicles obtained from PC12 cells pulse labeled with [35 S]sulfate were incubated for 15 min in nonaggregative milieu in the presence of 0.5 mg/ml of saponin ( Fig . 1, left) . This was the lowest saponin concentration that allowed the maximal release of the granins (N80% for SgII ; CgB was not quantitated because of the presence of a heparan sulfat.....
Document: The vast majority of the [35 S]sulfate-labeled granins were released into the supernatant when TGN vesicles obtained from PC12 cells pulse labeled with [35 S]sulfate were incubated for 15 min in nonaggregative milieu in the presence of 0.5 mg/ml of saponin ( Fig . 1, left) . This was the lowest saponin concentration that allowed the maximal release of the granins (N80% for SgII ; CgB was not quantitated because of the presence of a heparan sulfate proteoglycan (hsPG) in that region of the gel). The proportion of SgII released at a lower saponin concentration (0.25 mg/ml) did not increase when the incubation was carried out for up to 1 h (data not shown), indicating that for any given permeabilized TGN vesicle, the release of SgII occurred rapidly and was complete within 15 min .
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