Author: Falzarano, Darryl; de Wit, Emmie; Martellaro, Cynthia; Callison, Julie; Munster, Vincent J.; Feldmann, Heinz
Title: Inhibition of novel ß coronavirus replication by a combination of interferon-a2b and ribavirin Document date: 2013_4_18
ID: tgngoea8_19
Snippet: Genome quantification. RNA from AVL-treated supernatant was extracted with the NucleoSpin 96 Virus Core kit (Macherey-Nagel, Bethlehem, PA) on a Corbett Xtractor Gene (Valencia, CA). Quantitative real time RT-PCR using primers and probe previously described 48 was performed on the RotorGene Q (Qiagen). A 10-fold dilution series of viral RNA based on TCID 50 equivalents was used as a standard......
Document: Genome quantification. RNA from AVL-treated supernatant was extracted with the NucleoSpin 96 Virus Core kit (Macherey-Nagel, Bethlehem, PA) on a Corbett Xtractor Gene (Valencia, CA). Quantitative real time RT-PCR using primers and probe previously described 48 was performed on the RotorGene Q (Qiagen). A 10-fold dilution series of viral RNA based on TCID 50 equivalents was used as a standard.
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