Selected article for: "active site and Motif residue"
Author: Zhou, Yan; Zheng, HaiHong; Gao, Fei; Tian, DeBin; Yuan, ShiShan
Title: Mutational analysis of the SDD sequence motif of a PRRSV RNA-dependent RNA polymerase
  • Document date: 2011_9_16
    • ID: yo9libo0_41
    Snippet: There appears to be a strict requirement for the first aspartate, because any changes to this position, including a change to a negatively charged glutamate, are almost never tolerated for in vivo viral replication and/or in vitro RNA synthesis [1] . In the present study, all substitutions of the first aspartate residue of the SDD motif resulted in a lethal mutation. In agreement with previous studies [21] , the first aspartate residue of SDD is .....
    Document: There appears to be a strict requirement for the first aspartate, because any changes to this position, including a change to a negatively charged glutamate, are almost never tolerated for in vivo viral replication and/or in vitro RNA synthesis [1] . In the present study, all substitutions of the first aspartate residue of the SDD motif resulted in a lethal mutation. In agreement with previous studies [21] , the first aspartate residue of SDD is integral to the catalytic active site of the molecule and is absolutely conserved in the RdRp of PRRSV.

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