Author: Ding, Peiyang; Zhang, Teng; Li, Yafei; Teng, Man; Sun, Yaning; Liu, Xiao; Chai, Shujun; Zhou, Enmin; Jin, Qianyue; Zhang, Gaiping
Title: Nanoparticle orientationally displayed antigen epitopes improve neutralizing antibody level in a model of porcine circovirus type 2 Document date: 2017_7_24
ID: upb97on4_13
Snippet: The spatial structure of Cap conjugated to AuNPs was measured by enzyme-linked immunosorbent assay (ELISA). Cap protein and Cap-AuNPs were serially diluted 2-fold by carbonate buffer (pH 9.6), then coated on 96-well microtiter plates, and incubated at 4°C overnight. After blocking with skim milk (2.5% in PBST), monoclonal antibodies (mAbs) (stored in our lab) 9F4 and 6A5 with neutralizing ability and anti-His tags mAb and 8A10 mAb with anti-Cap .....
Document: The spatial structure of Cap conjugated to AuNPs was measured by enzyme-linked immunosorbent assay (ELISA). Cap protein and Cap-AuNPs were serially diluted 2-fold by carbonate buffer (pH 9.6), then coated on 96-well microtiter plates, and incubated at 4°C overnight. After blocking with skim milk (2.5% in PBST), monoclonal antibodies (mAbs) (stored in our lab) 9F4 and 6A5 with neutralizing ability and anti-His tags mAb and 8A10 mAb with anti-Cap (169-180) epitopes were added to the wells and then incubated at 37°C for 1 h, respectively. After washing with PBST five times, goat anti-mouse IgG conjugated to HRP was added for 1 h at 37°C. The reaction was developed by using 3,3,5,5-tetramethylbenzidine (TMB) as the substrate. The OD value of each well was measured at 450 nm using an ELISA reader.
Search related documents:
Co phrase search for related documents- Cap protein and carbonate buffer: 1
- Cap protein and ELISA immunosorbent assay: 1, 2
- Cap protein and ELISA reader: 1
- Cap protein and immunosorbent assay: 1, 2
- Cap protein and OD value: 1
- carbonate buffer and ELISA reader: 1, 2, 3, 4, 5
- carbonate buffer and HRP conjugate: 1
- carbonate buffer and immunosorbent assay: 1
- carbonate buffer and microtiter plate: 1
- carbonate buffer and OD value: 1
- ELISA immunosorbent assay and enzyme link: 1
- ELISA immunosorbent assay and enzyme link ELISA immunosorbent assay: 1
- ELISA immunosorbent assay and immunosorbent assay: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- ELISA immunosorbent assay and microtiter plate: 1, 2, 3
- ELISA immunosorbent assay and monoclonal antibody: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- ELISA immunosorbent assay and neutralize ability: 1
- ELISA immunosorbent assay and OD value: 1, 2, 3, 4, 5, 6
- ELISA reader and immunosorbent assay: 1, 2, 3, 4
- ELISA reader and OD value: 1
Co phrase search for related documents, hyperlinks ordered by date