Author: Xi, Hongbo; Tao, Weijian; Jian, Zhengguang; Sun, Xuefeng; Gong, Xiaohong; Huang, Lixin; Dong, Tianhua
Title: Levodopa attenuates cellular apoptosis in steroid-associated necrosis of the femoral head Document date: 2016_12_7
ID: z105jy9g_7
Snippet: Detection of cellular apoptosis. Paraffinized sections of the femoral head were used to study cellular apoptosis using a terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) kit (KGA703; KGI Biological Technology Development Co., Ltd., Nanjing, China), with 3,3'-daminobenzidine staining. Observed under the microscope, the nucleus was stained as tan or brown and set as the positive result. Five fields were randomly selected under x.....
Document: Detection of cellular apoptosis. Paraffinized sections of the femoral head were used to study cellular apoptosis using a terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) kit (KGA703; KGI Biological Technology Development Co., Ltd., Nanjing, China), with 3,3'-daminobenzidine staining. Observed under the microscope, the nucleus was stained as tan or brown and set as the positive result. Five fields were randomly selected under x400 magnification for observation, the proportion of apoptotic cells in 50% of the vision field were counted and indicated as the apoptosis index (AI).
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