Selected article for: "real time and threshold cycle"
Author: Yadana, Su; Coleman, Kristen Kelli; Nguyen, Tham Thi; Hansen-Estruch, Christophe; Kalimuddin, Shirin; Thoon, Koh Cheng; Low, Jenny Guek Hong; Gray, Gregory Charles
Title: Monitoring for airborne respiratory viruses in a general pediatric ward in Singapore
  • Document date: 2019_12_4
    • ID: ss9rc3bo_12
    Snippet: Eight (28.5%) of the 28 aerosol samples tested positive for adenovirus and one (3.5%) tested positive for influenza A virus. Aerosol samples with real-time RT-PCR/PCR cycle threshold (Ct) values <40 were considered positive. All eight adenovirus-positive samples were retrieved from the NIOSH samplers, 3 (37.5%) of which were from particles >4μm in aerodynamic diameter, and 5 (62.5%) from particles ≤4μm in aerodynamic diameter. The captured in.....
    Document: Eight (28.5%) of the 28 aerosol samples tested positive for adenovirus and one (3.5%) tested positive for influenza A virus. Aerosol samples with real-time RT-PCR/PCR cycle threshold (Ct) values <40 were considered positive. All eight adenovirus-positive samples were retrieved from the NIOSH samplers, 3 (37.5%) of which were from particles >4μm in aerodynamic diameter, and 5 (62.5%) from particles ≤4μm in aerodynamic diameter. The captured influenza A virus-positive particles were retrieved from a mobile SKC filter cassette and were therefore ≥0.3μm. None of the aerosol samples tested positive for influenza B or D virus, enterovirus or coronavirus. Attempts to grow viruses in cell culture from positive aerosol samples were unsuccessful.

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