Author: Ding, Peiyang; Zhang, Teng; Li, Yafei; Teng, Man; Sun, Yaning; Liu, Xiao; Chai, Shujun; Zhou, Enmin; Jin, Qianyue; Zhang, Gaiping
Title: Nanoparticle orientationally displayed antigen epitopes improve neutralizing antibody level in a model of porcine circovirus type 2 Document date: 2017_7_24
ID: upb97on4_68
Snippet: It is possible to improve the level of NAs by directly orienting antigens to nanoparticles and fully exposing neutralizing epitopes based on the analysis of the antigen structure. In the current study, we selected the Cap protein of PCV2 as a model antigen, which has immunodominant epitopes and neutralizing epitopes. We conjugated Cap to AuNPs by the Au-thiol interaction, which was confirmed by FTIR, resulting in the immunodominant epitopes being.....
Document: It is possible to improve the level of NAs by directly orienting antigens to nanoparticles and fully exposing neutralizing epitopes based on the analysis of the antigen structure. In the current study, we selected the Cap protein of PCV2 as a model antigen, which has immunodominant epitopes and neutralizing epitopes. We conjugated Cap to AuNPs by the Au-thiol interaction, which was confirmed by FTIR, resulting in the immunodominant epitopes being obscured while fully exposing the neutralizing epitopes on the outer surface. AuNPs promoted phagocytosis of Cap protein by APCs without inducing inflammatory cytokine secretion, enhanced humoral and cellular immunity, and balanced Th1 and Th2 type cellular immunity. The NAs were significantly improved, while antibody against the immunodominant epitope was significantly reduced. Overall, this study demonstrates that the strategy of nanoparticles orientationally displaying antigen epitopes appears to be feasible for vaccine development.
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