Author: Zhou, Yan; Zheng, HaiHong; Gao, Fei; Tian, DeBin; Yuan, ShiShan
Title: Mutational analysis of the SDD sequence motif of a PRRSV RNA-dependent RNA polymerase Document date: 2011_9_16
ID: yo9libo0_25
Snippet: vGDD in vitro generated from MARC-145 cells were serially 10-fold diluted. 0.2 mL viruses was inoculated onto MARC-145 monolayers (8×10 5 cells/well) in 6-well plates. After 1 h at 37°C, cell monolayers were washed once with PBS and overlaid with 3 mL MEM containing 1% (w/v) low melting-point agarose and 2% FBS. After incubation for further four days at 37°C, single plaques were selected and dissolved into 200 ïL 2% MEM, and then inoculated .....
Document: vGDD in vitro generated from MARC-145 cells were serially 10-fold diluted. 0.2 mL viruses was inoculated onto MARC-145 monolayers (8×10 5 cells/well) in 6-well plates. After 1 h at 37°C, cell monolayers were washed once with PBS and overlaid with 3 mL MEM containing 1% (w/v) low melting-point agarose and 2% FBS. After incubation for further four days at 37°C, single plaques were selected and dissolved into 200 ïL 2% MEM, and then inoculated onto MARC-145 cells for producing viruses.
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