Author: Zhou, Yan; Zheng, HaiHong; Gao, Fei; Tian, DeBin; Yuan, ShiShan
Title: Mutational analysis of the SDD sequence motif of a PRRSV RNA-dependent RNA polymerase Document date: 2011_9_16
ID: yo9libo0_8
Snippet: The PCR products were purified using the Watson Gel Extraction Mini Kit (Watson, Shanghai, China). Purified products were digested with Avr II and EcoR V and the major fragment was used to replace the Avr II/EcoR V fragment of the infectious clone pAPRRS to form the mutant clones......
Document: The PCR products were purified using the Watson Gel Extraction Mini Kit (Watson, Shanghai, China). Purified products were digested with Avr II and EcoR V and the major fragment was used to replace the Avr II/EcoR V fragment of the infectious clone pAPRRS to form the mutant clones.
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