Author: Imai-Matsushima, Aki; Martin-Sancho, Laura; Karlas, Alexander; Imai, Seiichiro; Zoranovic, Tamara; Hocke, Andreas C.; Mollenkopf, Hans-Joachim; Berger, Hilmar; Meyer, Thomas F.
Title: Long-Term Culture of Distal Airway Epithelial Cells Allows Differentiation Towards Alveolar Epithelial Cells Suited for Influenza Virus Studies Document date: 2018_6_22
ID: vopbw1tl_30
Snippet: After removal of feeders, qRT-PCR analysis showed expression of surfactant-associated protein A (SFTPA) (Fig. 5E) and AQP5 (Fig. 5F) , key markers of chicken pneumocytes. In contrast to human cells, chicken DAECs did not require additional factors to induce these genes, and their expression level remained relatively stable over time (Fig. 5E, F) ......
Document: After removal of feeders, qRT-PCR analysis showed expression of surfactant-associated protein A (SFTPA) (Fig. 5E) and AQP5 (Fig. 5F) , key markers of chicken pneumocytes. In contrast to human cells, chicken DAECs did not require additional factors to induce these genes, and their expression level remained relatively stable over time (Fig. 5E, F) .
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