Selected article for: "µg ml and viable cell"
Author: Wu, Beibei; Wang, Liyin; Jiang, Lili; Dong, Lili; Xu, Fengli; Lu, Yili; Jin, Jiahui; Wang, Zhanyue; Liang, Guang; Shan, Xiaoou
Title: n-butanol extract from Folium isatidis inhibits the lipopolysaccharide-induced downregulation of CXCR1 and CXCR2 on human neutrophils
  • Document date: 2017_10_25
    • ID: w85t4zz6_10
    Snippet: Quantification of IL-8. The neutrophils were pretreated for 2 h with the extract as described above, and then stimulated with LPS (0.5 µg/ml) for 1 or 4 h. The supernatant and cells were collected, and the levels of IL-8 in the supernatant were determined using enzyme-linked immunosorbent assay (ELISA) kits (cat no. 88-8086-88; eBioscience; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. The total quantity of IL-8 in th.....
    Document: Quantification of IL-8. The neutrophils were pretreated for 2 h with the extract as described above, and then stimulated with LPS (0.5 µg/ml) for 1 or 4 h. The supernatant and cells were collected, and the levels of IL-8 in the supernatant were determined using enzyme-linked immunosorbent assay (ELISA) kits (cat no. 88-8086-88; eBioscience; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. The total quantity of IL-8 in the supernatant was normalized to that of total protein in the viable cell pellets.

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