Selected article for: "genome amplification and positive control"

Author: Baldwin, Don A.; Feldman, Michael; Alwine, James C.; Robertson, Erle S.
Title: Metagenomic Assay for Identification of Microbial Pathogens in Tumor Tissues
  • Document date: 2014_9_16
  • ID: xlqdn0c7_10
    Snippet: Assay response to positive controls. The limited amounts of tissue available in most tumor archives or obtained from clinical procedures, such as fine needle aspirates and other biopsy specimens, require that metagenomic screening protocols include efficient strategies for nucleic acid extractions combined with an amplification step which allows for genome-or transcriptome-wide representation of microbial agents present in the sample. These metho.....
    Document: Assay response to positive controls. The limited amounts of tissue available in most tumor archives or obtained from clinical procedures, such as fine needle aspirates and other biopsy specimens, require that metagenomic screening protocols include efficient strategies for nucleic acid extractions combined with an amplification step which allows for genome-or transcriptome-wide representation of microbial agents present in the sample. These methods must additionally be compatible with the degraded DNA and RNA typically produced by formalin tissue fixation. A draft workflow was designed to address these technical hurdles (Fig. 1C) , and pilot experiments were conducted to test the amplification and detection of a number of positive-control viruses.

    Search related documents:
    Co phrase search for related documents
    • amplification step and nucleic acid extraction: 1, 2, 3, 4
    • amplification step and pilot experiment: 1
    • amplification step and positive control: 1, 2, 3, 4
    • Assay response and detection amplification: 1
    • Assay response and positive control: 1, 2, 3, 4
    • available tissue and tissue fixation: 1
    • clinical procedure and nucleic acid extraction: 1
    • clinical procedure and screening protocol: 1, 2, 3